《植物生理学报》 2015, 51(6): 840-846

碳酸钠胁迫对甜菜幼苗生长及保护酶的影响

郭剑, 李彩凤*, 陈明, 徐影, 刘磊, 桑丽敏, 盖志佳, 王玉波, 曹英雪, 马凤鸣

东北农业大学农学院, 哈尔滨150030

通信作者：李彩凤；E-mail: licaifeng@neau.edu.cn；Tel: 0451-55190854

摘　要：

以甜菜‘KWS0143’和‘Beta464’两个品种为试验材料, 研究不同浓度(0、0.4%、0.8%和1.2%) Na₂CO₃胁迫下, 甜菜苗期植株生长, 叶片及根系的超氧化物歧化酶(SOD)、过氧化物酶(POD)及丙二醛(MDA)的变化。结果表明, 同一取样时期, 随着Na₂CO₃浓度的增加, 植株的干鲜重呈先增加后降低的趋势, 0.4% Na₂CO₃处理下甜菜幼苗干鲜重显著高于对照(P<0.05)。叶片和根中的SOD和POD活性变化趋势相一致, 总体呈先升高后下降的趋势, 在0.4% Na2CO3处理下活性最高。MDA含量则呈递增的趋势, 1.2% Na₂CO₃处理下含量最多。同一浓度下, SOD和POD活性随着处理时间的延长呈先上升后下降的趋势, 且在2对真叶展开后的第3周活性最高, 而MDA含量则呈现急剧增加后缓慢上升的趋势。而且高浓度1.2% Na₂CO₃处理下‘KWS0143’耐盐碱能力强于‘Beta464’。

关键词：甜菜; 碳酸钠胁迫; 过氧化物酶; 超氧化物歧化酶; 丙二醛

收稿：2014-12-26   修定：2015-04-29

资助：国家自然科学基金(31171493)。

Effects of Na₂CO₃ Stress on the Growth and Antioxidant Enzymes of Beta vulgaris Seedlings

GUO Jian, LI Cai-Feng*, CHEN Ming, XU Ying, LIU Lei, SANG Li-Min, GAI Zhi-Jia, WANG Yu-Bo, CAO Ying-Xue, MA Feng-Ming

College of Agricultural, Northeast Agricultural University, Harbin 150030, China

Corresponding author: LI Cai-Feng; E-mail: licaifeng@neau.edu.cn; Tel: 0451-55190854

Abstract:

The sugar beet (Beta vulgaris) ‘KWS0143’ and ‘Beta464’ were used as materials, to study the activities of SOD, POD and the content of MDA in leaves and roots under different concentrations (0, 0.4%, 0.8%, 1.2%) of Na₂CO₃ stress. The results showed that in same sampling period, fresh and dry weight of plants increased firstly and then decreased with increasing concentration of Na₂CO₃, and the fresh and dry weight of seedlings with 0.4% Na₂CO₃ treatment were significantly higher than those of CK. The activities of SOD and POD in leaves and roots had the same tends which increased at primary stage and then decreased, and the activities were the highest with 0.4% Na₂CO₃ treatment. The content of MDA increased and reached the highest with 1.2% Na₂CO₃ in leaves and roots. In the same concentration, the activities of SOD and POD increased firstly and then decreased with the extension of time.In the third week after the two pairs of true leaves unfolded, the activities reached highest. But the content of MDA increased slowly after a sharp upward trend over time. It indicated that under 1.2% Na₂CO₃ stress the salinity ability of ‘KWS0143’ was more stronger than ‘Beta464’.

Key words: Beta vulgaris (sugar beet); Na₂CO₃ stress; POD; SOD; MDA